Use of Escherichia coli Nissle 1917 producing recombinant colicins for treatment of IBD patients Roman Kotłowski. Med Hypotheses. 2016 Aug. Abstract Patients with Crohn's Disease and Ulcerative Colitis infected with Adherent-Invasive Escherichia coli strains constitute the largest group among Inflammatory Bowel Disease subjects, when taking into account all known etiological agents of the disease. A possible link between these pathogenic bacteria and inflammation process has gained the confidence in recently published papers. Observed enteric neuroglial cells apoptosis and epithelial gaps of ileum are probably the key manifestations of inflammation, which has been shown in IBD patients in contrary to the samples taken from healthy individuals. The cascade of consecutive events from bacterial infection via inflammation to excessive apoptosis in IBD patients leads up to the aim of our hypothesis about designing of new therapeutic strategy directed to Adherent-Invasive E. coli strains. The main advantage of biological method, which will rely on application of E. coli Nissle 1917 strain as a carrier for specific recombinant colicins against AIEC strains, could probably cause a long-lasting remission of inflammation in CD and UC patients. Copyright © 2016 Elsevier Ltd. All rights reserved. Similar articles Point mutations in FimH adhesin of Crohn's disease-associated adherent-invasive Escherichia coli enhance intestinal inflammatory response. Dreux N, Denizot J, Martinez-Medina M, Mellmann A, Billig M, Kisiela D, Chattopadhyay S, Sokurenko E, Neut C, Gower-Rousseau C, Colombel JF, Bonnet R, Darfeuille-Michaud A, Barnich N. Dreux N, et al. PLoS Pathog. 2013 Jan;9(1):e1003141. doi: Epub 2013 Jan 24. PLoS Pathog. 2013. PMID: 23358328 Free PMC article. Inflammation-associated adherent-invasive Escherichia coli are enriched in pathways for use of propanediol and iron and M-cell translocation. Dogan B, Suzuki H, Herlekar D, Sartor RB, Campbell BJ, Roberts CL, Stewart K, Scherl EJ, Araz Y, Bitar PP, Lefébure T, Chandler B, Schukken YH, Stanhope MJ, Simpson KW. Dogan B, et al. Inflamm Bowel Dis. 2014 Nov;20(11):1919-32. doi: Inflamm Bowel Dis. 2014. PMID: 25230163 Invasive Escherichia coli are a feature of Crohn's disease. Sasaki M, Sitaraman SV, Babbin BA, Gerner-Smidt P, Ribot EM, Garrett N, Alpern JA, Akyildiz A, Theiss AL, Nusrat A, Klapproth JM. Sasaki M, et al. Lab Invest. 2007 Oct;87(10):1042-54. doi: Epub 2007 Jul 30. Lab Invest. 2007. PMID: 17660846 Activity of Species-specific Antibiotics Against Crohn's Disease-Associated Adherent-invasive Escherichia coli. Brown CL, Smith K, Wall DM, Walker D. Brown CL, et al. Inflamm Bowel Dis. 2015 Oct;21(10):2372-82. doi: Inflamm Bowel Dis. 2015. PMID: 26177305 [Crohn disease, ulcerative colitis. When bacteria attack the intestinal wall....]. Duchmann R, Lochs H, Kruis W. Duchmann R, et al. MMW Fortschr Med. 1999 Dec 16;141(51-52):48-51. MMW Fortschr Med. 1999. PMID: 10949626 Review. German. Cited by Efficient markerless integration of genes in the chromosome of probiotic E. coli Nissle 1917 by bacterial conjugation. Seco EM, Fernández LÁ. Seco EM, et al. Microb Biotechnol. 2022 May;15(5):1374-1391. doi: Epub 2021 Nov 9. Microb Biotechnol. 2022. PMID: 34755474 Free PMC article. Adherent-Invasive E. coli: Update on the Lifestyle of a Troublemaker in Crohn's Disease. Chervy M, Barnich N, Denizot J. Chervy M, et al. Int J Mol Sci. 2020 May 25;21(10):3734. doi: Int J Mol Sci. 2020. PMID: 32466328 Free PMC article. Review. New Approaches for Escherichia coli Genotyping. Kotłowski R, Grecka K, Kot B, Szweda P. Kotłowski R, et al. Pathogens. 2020 Jan 21;9(2):73. doi: Pathogens. 2020. PMID: 31973175 Free PMC article. K5 Capsule and Lipopolysaccharide Are Important in Resistance to T4 Phage Attack in Probiotic E. coli Strain Nissle 1917. Soundararajan M, von Bünau R, Oelschlaeger TA. Soundararajan M, et al. Front Microbiol. 2019 Nov 29;10:2783. doi: eCollection 2019. Front Microbiol. 2019. PMID: 31849915 Free PMC article. Integrating omics for a better understanding of Inflammatory Bowel Disease: a step towards personalized medicine. Kumar M, Garand M, Al Khodor S. Kumar M, et al. J Transl Med. 2019 Dec 13;17(1):419. doi: J Transl Med. 2019. PMID: 31836022 Free PMC article. Review. MeSH terms Substances LinkOut - more resources Full Text Sources ClinicalKey Elsevier Science Other Literature Sources scite Smart Citations Medical MedlinePlus Health Information
The probiotic strain Escherichia coli Nissle 1917 (EcN) is a common bacterial chassis in synthetic biology developments for therapeutic applications given its long track record of safe administration in humans. Chromosomal integration of the genes of interest (GOIs) in the engineered bacterium offer …Escherichia coli (E. coli) E. coli are mostly harmless bacteria that live in the intestines of people and animals and contribute to intestinal health. However, eating or drinking food or water
Escherichia coli strain Nissle 1917 ameliorates experimental colitis by modulating intestinal permeability, the inflammatory response and clinical signs in a faecal transplantation model. J Med Microbiol. 2016; 65 (3):201–210. doi: 10.1099/jmm.0.000222. [Google Scholar]
Confluent monolayers of IPEC-J2 cells were pre-incubated with E. coli Nissle 1917 (EcN), E. coli 140815 or E. coli MG1655 at an MOI of 100∶1 or 10∶1 bacteria to host cells. After two hours, cells were washed and infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells.
